Quantification of Gentamicin by microbial assay technique and reverse phase HPLC
نویسندگان
چکیده
The preliminary research work carried to find out the concentration of Gentamicin in the raw material. Two different techniques used in this study for the quantification of Gentamicin. They are microbiological assay method and reversed phase HPLC. The concentration of Gentamicin was quantified even at very lower concentration by microbiological assay method and it is sensitive. The test organisms used is Staphylococcus epidermidis and Bacillus pumilus. The components present in Gentamicin were estimated by reverse phase HPLC was found to be C1, C1a, C2a and C2. It is therefore concluded that the microbial assay used for assay purpose and HPLC is used for estimating the components present in Gentamicin. This study can be further carried out for the development of Gentamicin by HPLC method as a prolonged research work.
منابع مشابه
Determination of gentamicin in different matrices by a new sensitive high-performance liquid chromatography-mass spectrometric method.
OBJECTIVES The aim of this work was to develop and validate an HPLC method for gentamicin quantification in different types of biological samples such as animal tissues and cellular material and also in pharmaceuticals. METHODS Poly(lactide-co-glycolide) microparticles (MP) of gentamicin (PLGA 502H MP), THP-1 cells, and plasma and tissue samples of mice treated with the antibiotic either free...
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